Examinando por Materia "FIBROBLASTOS"

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    PublicaciónAcceso abierto
    Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel
    (2014-05-05) Valencia-Serna, J. (Juliana); Pineda-Molina, C. (Catalina)
    Wounds can cause loss of skin that limits the skin regeneration, making the treatment more difficult to address. The tissue engineering has developed skin substitutes that promote skin regeneration; however, it is imperative to find materials that allow fibroblast growth in order to find an appropriate skin substitute. The construction of tridimensional porous collagen and collagenhyaluronic acid matrixes crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride is presented in order to evaluate their potential use in skin regeneration therapies. Matrix porosity was evaluated. Degradation tests, human fibroblast adherence, viability and proliferation tests were performed. Matrixes had a uniform distributed porosity with mean diameters of 50 μm. Both collagen and collagen-hyaluronic acid (2:8 and 4:6) matrixes presented a progressive degradation rate with similar weight. After 24 hours under culture, the number of fibroblasts seeded on collagen matrixes were doubled, while the number of fibroblast seeded on collagen-hyaluronic acid matrixes remained similar. Even though porous structure and degradation rate of different types of constructed matrixes in this study are similar, collagen matrixes offer better adhesion and proliferation conditions for seeded fibroblasts in comparison with hyaluronic acid added matrixes, making them the best scaffold to be employed as a dermic substitute among the matrixes compared here.
  • Miniatura
    PublicaciónAcceso abierto
    Soluciones terapéuticas para la reconstrucción de la dermis y la epidermis. Oportunidades en el medio antioqueño
    (2014-04-23) Velásquez-Puerta, D. (Diego); Pineda-Molina, C. (Catalina); Cardona-Cano, M (María); Gómez-Suárez, N. E. (Nicolás Eugenio); Gartz-Moises, G. (Grete); Úsuga-Gómez, I. (Isabel); Tróchez-Wilchez, D. (Diana); Londoño-Peláez, C. (Carolina)
    In Antioquia (Colombia), there are many patients who suffer dermal injuries caused by accident or disease. Therefore, it is necessary to pursue available, effective and affordable solutions to overcome dermal problems. Physicians and patients are both expecting corrective treatments that can reduce inner tissue exposition and allow the possibility of recovering skin functionality. Although there are many solutions for skin replacement, some obstacles remain, such as: high costs, limited availability of skin for implantation in extended areas, adequate functionality of the restored tissue and immunological rejection. Due to these issues, this article presents some of the existing alternative solutions for patients with skin injuries, considering the histology and physiology of the skin, and regarding the classical and economically attainable solutions to be used in Antioquia.