Examinando por Materia "CÉLULAS MADRE MESENQUIMATOSAS"

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    Isolation of human bone marrow mesenchymal stem cells and evaluation of their osteogenic potential
    (2014-04-23) García-Quiroz, F. (Felipe); Posada-Estefan, O. (Olga); Gallego Pérez, D. (Daniel); Higuita-Castro, N. (Natalia); Sarassa-Velásquez, C. (Carlos); Hansford, D. (Derek); Agudelo-Florez, P. (Piedad); López-Rojas, L. E. (Luis Ernesto)
    Human bone marrow mesenchymal stem cells (hBMSCs) comprise a cell population capable of self-renewal and multilineage differentiation commonly isolated from bone marrow aspirates of large bones. Their osteogenic potential has been extensively exploited for the biological evaluation of scaffolds or biomaterials with applications in bone tissue engineering. This work aimed to isolate hBMSCs from femoral heads of patients undergoing total hip arthroplasty and to evaluate their osteogenic potential. Briefly, the trabecular bone was extracted and mechanically disaggregated; the released cells were cultured and non-adherent cells were removed after 4 days. The osteogenic potential was evaluated at the fifth passage after 14 and 20 days of induction, comparing cultures with and without osteogenic supplements, via Alizarin red staining and the quantification of the gene expression levels of the osteogenic markers collagen type I, osteonectin and bone sialoprotein through real-time RT-PCR. The obtained hBMSCs presented a stable undifferentiated phenotype after prolonged cell culture, matrix mineralization capabilities and expression of osteoblast phenotype upon osteogenic induction. The three markers were up-regulated in cultures under osteogenic conditions and 2 fold differences in their expression levels were found to be significant for the onset of the differentiation process. The obtained hBMSCs may have applications on the in vitro evaluation of the osteoinductivity of different biomaterials, bioactive molecules or tissue engineering scaffolds.
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    Obtención de células madre del tejido adiposo y su potencial de diferenciación ontogénico
    (2014-04-23) Pineda-Molina, C. (Catalina); Londoño-Peláez, C. (Carolina)
    Adipose tissue is one of the main sources of stem cells readily available and with a high potential to differentiate into specifi c mesenchymal and non-mesenchymal lineages. Therefore, to standardize the collection of these cells, adipose tissue from surgical liposuctions and abdominoplasty was used. Methods of enzymatic and mechanical digestion were used to release the cells. With regard to cell morphology, the cells displayed a fi broblast-like spindleshaped morphology as cited by other authors. There was a higher effi ciency in processing tissue from liposuctions compared with tissue from abdominoplasty. Likewise, the enzymatic digestion allowed faster initial cell adhesion than the mechanical digestion. Finally, calcium deposits were observed by alizarin red staining in cultures under osteogenic conditions, but were absent in cultures lacking osteogenic supplements. In conclusion, isolated adiposedderived stem cells were capable of osteogenic differentiation and therefore with potential applications in bone tissue engineering.