Browsing by Author "Zapata-Ocampo, P. A. (Paola Andrea)"

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    PublicationOpen Access
    Producción de biomasa y exopolisacáridos de grifola frondosa bajo cultivo sumergido utilizando fuentes de carbono no convencionales
    (2014-05-08) Zapata-Ocampo, P. A. (Paola Andrea); Rojas-Vahos, D. F. (Diego Fernando); Fernández-Ossa, C. E. (Carlos Eduardo); Ramirez-Cadavid, D. A. (David Alexander); Restrepo, G. (Gloria); Orjuela, V. (Viviana); Arroyave, M. (Marcela); Gómez, T. (Tatiana); Atehortúa-Garcés, L. (Lucia)
    Grifola frondosa is a Basidiomycete fungus edible and medicinal, it produces polysaccharides such a glucans (β-1,6 and β-1,3) with anti-tumor and inmuno-stimulating activity. For this work, the aim was evaluate the incidence of different carbon sources (FC) in biomass and exo-polymer (EPS) production, under submerged culture conditions, by using a factorial statistical design with two factors: carbon source (FC(1-7)) and medium (MB and MS). The maximal yield of mycelial biomass (21,10 ± 0,82) g/l and the maximal yield in EPS (6,53 ± 0,14) g/l was achieved by using the MB medium supplemented with FC4. Besides, it was possible to develop a medium that fulfills the objective of reducing costs from $18.455 to $656 Colombian pesos for each liter of medium used, which produces (14,14 ± 0,82) g/l of biomass and (5,16 ± 0,14) g/l of EPS, using the MS medium supplemented with FC4. This result opens new alternatives and possibilities in our country for developing products of this species with cost that can be reached by population.
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    PublicationOpen Access
    Producción de matrices de quitosano extraído de crustáceos
    (2014-04-30) Escobar-Sierra, D. M. (Diana Marcela); Urrea-Llano, C. A. (Carlos Alberto); Gutiérrez-Guerra, M. (Mariana); Zapata-Ocampo, P. A. (Paola Andrea)
    Chitosan is present in crustacean shells and it has been used in the fields of medicine and tissue engineering for the construction of scaffolds that support cell growth. In this study, chitosan was extracted from crustacean shells and processed into scaffolds with controlled microstructure using a simple processing method presented herein. The scaffolds were prepared by freezing and lyophilization of chitosan solutions and were characterized by scanning electron microscopy. The results showed a chitosan with an X-ray diffraction spectrum similar to that of a commercial chitosan, thus demonstrating the effectiveness of the extraction protocol. Microscopy showed oval and circular pores distributed on the bulk sample, with pore diameters between 100 μm and 150 μm. This shows that the proposed fabrication method provides a starting point for the construction of porous scaffolds that may support cell growth.