La Biotecnología y el desarrollo de investigación aplicada en el programa de Ingeniería Biomédica de la EIA y el CES

López-Rojas, L. E. (Luis Ernesto) | 2014-04-20

Obtención de células madre del tejido adiposo y su potencial de diferenciación ontogénico

Pineda-Molina, C. (Catalina) | 2014-04-23

Adipose tissue is one of the main sources of stem cells readily available and with a high potential to differentiate into specifi c mesenchymal and non-mesenchymal lineages. Therefore, to standardize the collection of these cells, adipose tissue from surgical liposuctions and abdominoplasty was used. Methods of enzymatic and mechanical digestion were used to release the cells. With regard to cell morphology, the cells displayed a fi broblast-like spindleshaped morphology as cited by other authors. There was a higher effi ciency in processing tissue from liposuctions compared with tissue from abdominoplasty. Likewise, the enzymatic digestion allowed faster initial cell adhesion than the mechanical digestion. Finally, calcium deposits were observed by alizarin red staining in cultures under osteogenic conditions, but were absent in cultures lacking osteogenic supplements. In conclusion, isolated adiposedderived stem cells were capable of osteogenic differentiation and therefore with potential applications in bone tissue engineering.

Reflexión: sostenibilidad y procesos de calidad en una revista científica latinoamericana

García-Quiroz, F. (Felipe) | 2014-04-23

Soluciones terapéuticas para la reconstrucción de la dermis y la epidermis. Oportunidades en el medio antioqueño

Velásquez-Puerta, D. (Diego) | 2014-04-23

In Antioquia (Colombia), there are many patients who suffer dermal injuries caused by accident or disease. Therefore, it is necessary to pursue available, effective and affordable solutions to overcome dermal problems. Physicians and patients are both expecting corrective treatments that can reduce inner tissue exposition and allow the possibility of recovering skin functionality. Although there are many solutions for skin replacement, some obstacles remain, such as: high costs, limited availability of skin for implantation in extended areas, adequate functionality of the restored tissue and immunological rejection. Due to these issues, this article presents some of the existing alternative solutions for patients with skin injuries, considering the histology and physiology of the skin, and regarding the classical and economically attainable solutions to be used in Antioquia.